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Click Add Plate Read to Step to designate when fluorescence data will be acquired during the protocol. Click Insert Step to add a temperature step to the protocol.Ĭlick Delete Step to remove a highlighted step from the protocol.ģ. Click on the temperature or dwell time to directly edit the value.Ģ. Select any step in either the graphical or text display - the step becomes highlighted in blue. The Protocol Editor is used to create a new protocol or edit an existing one (Figure 2).ġ. Use the Express Load drop-down menu to directly load a protocol to use in an experiment or to edit.Ĭlick Edit Selected to open the Protocol Editor to edit the steps of the selected protocol.Ĭlick the Start Run tab to proceed and run an experiment with the currently loaded protocol. To load a protocol to use in an experiment or to edit. The Protocol tab displays a preview of the protocol loaded in the Experiment Setup window (Figure 1).Ĭlick Create New to open the Protocol Editor to create a new protocol.Ĭlick Select Existing to launch the file browser

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